Akershus Universitetssykehus Hf

Oslo

Runar Almaas

Avdelingsleder

<p>We announce 80%, one-year extendable researcher position in the CRISPR gene therapy group led by Emma Haapaniemi to be based at NCMBM, University of Oslo &amp; Oslo University Hospital. We welcome applications both from recent PhD graduates and more experienced scientists.   <br></p><p>The successful candidate will join a highly integrated and motivated team with diverse skills and expertise across molecular biology and immunology. The work tasks will concentrate on optimizing and scaling up protocols for efficient mutation correction in human T cells and hematopoietic stem cells utilizing CRISPR-Cas9 gene editing technologies. Examples of work tasks include optimization of patient-derived T cell and stem cell ex vivo editing &amp; culturing conditions; establishing functional assays; design and optimizing high-throughput biology experiments in robotics platforms; setting up custom Next Generation Sequencing libraries and technologies; scale up protocols to pre-GMP and GMP scale</p><p><strong>About the laboratory</strong> <br></p><p>The aim of the Haapaniemi group (www.haapaniemilab.org) is to build a gene editing platform that can be used to correct patient-specific mutations in ex vivo cell therapies for genetic immune and other rare childhood diseases. We use high-throughput biology techniques to optimize, develop and understand the CRISPR-Cas9 system that forms the base of the platform, and develop and implement methods to evaluate its safety in a clinical setting. We will use gene editing technologies for the benefit of the patients in Nordic hospitals.  <br><strong>Norwegian Centre for Molecular Biosciences and Medicine (NCMBM)</strong> was established in 2008 and is the Norwegian node in the Nordic EMBL Partnership for Molecular Medicine. NCMM is a joint venture between the University of Oslo, Health Region South-East and the Research Council of Norway, and has altogether 11 research groups. The overall objective of NCMM is to conduct cutting edge research in molecular medicine and biotechnology as well as facilitate translation of discoveries in basic medical research into clinical practice.</p><em>Oslo University Hospital is a workplace with great diversity. We believe this is crucial for solving our tasks in the best possible way. We want this diversity to be reflected among the applicants for our positions, and we encourage all qualified candidates, regardless of background, to apply!</em>
<h2>Arbeidsoppgaver</h2>
<ul><li>Design and conduct custom CRISPR experiments and screens in high-throughput robotics platforms</li><li>Set up custom Next Generation Sequencing libraries and technologies<br></li><li>Set up functional assays for immune cells<br></li><li>Supervise students and technicians in designing and conducting molecular biology experiments      </li></ul>
<h2>Kvalifikasjoner</h2>
<ul><li>PhD in molecular biology, biology or immunology with researcher/ postdoctoral researcher experience of min 4 years  <br></li><li>Documented experience with the following techniques: previous experience in CRISPR-Cas9 gene editing in healthy and patient-derived T cells, Next Generation Sequencing library preparation, immune cell culture, flow cytometry and droplet digital PCR</li><li>At least 3 published first-author papers and 3 as a last/senior author <br></li><li>Experience in supervising interns, masters and PhD students (both co- and main supervisor)<br></li><li>Experience in genetic analyses<br></li><li>Fluent oral and written English communication skills</li></ul><p>&nbsp;</p><p><strong>The application must include:</strong> <br></p><ul><li>Cover letter (statement of motivation, summarizing scientific work      and research interest)  <br></li><li>CV (summarizing education, positions, pedagogical or      administrative experience and other qualifying activity)  <br></li><li>Copies of educational certificates (academic transcripts only)  <br></li><li>List of publications  <br></li><li>List of 3 reference people (name, relation to candidate, e-mail      and phone number) </li></ul>
<h2>Personlige egenskaper</h2>
<ul><li>Organized and independent<br></li><li>Ability to communicate and work in shared multidisciplinary projects<br></li><li>Willingness to supervise students <br></li><li>Enthusiastic and hard-working scientist       </li></ul>
<h2>Vi tilbyr</h2>
<ul><li>Salary depending on qualifications <br></li><li>An exciting, translational project where your strong basic science background can directly benefit our sickest patients<br></li><li>Attractive welfare benefits and a generous pension agreement, in addition to Oslo’s family-friendly environment with its rich opportunities for culture and outdoor activities    </li></ul>

Researcher in CRISPR gene therapy group